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NHPX transiently accumulates in splicing speckles. Microinjection of pAL107EYFP–NHPX into (A) transformed cell line MCF7 and (B) primary fibroblast htert1787. The microinjected cells were fixed at different time points (1 h, 6 h and 16 h) and counterstained with anti-SC35 to denote speckles and anti-coilin 204/10 to denote CBs. Arrowheads indicate CBs, arrows indicate nucleoli and broken arrows indicate speckles. Bars, 5 μm.
