Table 1.
Primer sequences used for real-time PCR
| Target mRNA | Forward primer (5'→ 3') | Reverse primer (5'→ 3') | Product size (bp) |
| β-actin | TGACAGGATGCAGAAGGAGA | CGCTCAGGAGGAGCAATG | 75 |
| ALP | AAGGCTTCTTCTTGCTGGTG | GCCTTACCCTCATGATGTCC | 61 |
| ATF4 | ATGATGGCTTGGCCAGTG | TCTCCAACATCCAATCTGTCC | 72 |
| Cbfa1 | GATGACACTGCCACCTCTGA | GCCCAGTTCTGAAGCACCT | 81 |
| M-CSF | CAGCTGCTTCACCAAGGACT | TCATGGAAAGTTCGGACACA | 60 |
| OCN | AGACTCCGGCGCTACCTT | CTCGTCACAAGCAGGGTTAAG | 93 |
| OPG | ATGAACAAGTGGCTGTGCTG | CAGTTTCTGGGTCATAATGCAA | 106 |
| Osterix | TTTAAACAAACACGATGATGATGA | ATTGGACTTCCCCCTTCTTG | 138 |
| RANKL | TGAAGACACACTACCTGACTCCTG | CCACAATGTGTTGCAGTTCC | 83 |