Figure 1. Nucleotide and translated amino acid sequences for variable regions.
(a) The light chain variable region of 13F6-1-2 compared with the BALB/c Vλx germline (GenBank accession number M34597), and the Vλx-light chains F28C4 (GenBank accession number X76752) and MW1 (GenBank accession number M34598). 13F6-1-2 contains two mutations from the Vλx germline: a Ser to Arg mutation at residue 27 in CDR L1 and a Lys to Arg mutation in framework residue 45. (b) The heavy chain variable region of 13F6-1-2 compared with the germline VH283 and F28C4. The amino acids are labeled according to the Kabat numbering scheme and hypervariable loops are highlighted in colour.