Figure 6. Similarity of 13F6-1-2 Vλx and human germline Vλ subtype VIII light chains.
(a) Sequence alignment of the 13F6-1-2 Vλx light chain with human germline Vλ subtype VIII light chain (GenBank accession number L29806). (b) Three framework residues of 13F6-1-2 interact with the CDRs and may need to be maintained in humanization. Asp L70 hydrogen bonds to Thr L23 in CDR L1, Ser L105 hydrogen bonds to Glu L49 of CDR L2, and Tyr L36 interacts with Tyr L96 from CDR L3.