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. 2003 Sep 29;162(7):1281–1292. doi: 10.1083/jcb.200304018

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Copyright © 2003, The Rockefeller University Press

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Figure 8. — Dominant-negative RhoA substantially abolishes the inhibitory effect of ephrin-A1 on cell protrusions. Expression of dominant-negative and dominant-active RhoA and Rac1 in MDCK cells was induced by removing tetracycline from the culture medium for 2 d. Branching morphogenesis assays were conducted as described in Materials and methods. After a 24-h stimulation with HGF in the presence of ephrin-A1–Fc or Fc, cells with membrane processes longer than the diameters of cell bodies were counted. Values represent means from six randomly selected fields, ± SD.