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. 2002 Dec 23;159(6):1029–1037. doi: 10.1083/jcb.200207165

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Copyright © 2002, The Rockefeller University Press

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Figure 1. — Ghrelin protects H9c2 cardiomyocytes from doxorubicin-induced cell death. H9c2 cardiomyocytes were treated with 1 μM doxorubicin (grey bar) in the presence or absence of 1 μM ghrelin. (A) Phase–contrast images (200×; bar, 0.1 mm) after 24 h of treatment: untreated (a), ghrelin (b), doxorubicin (c), and doxorubicin + ghrelin (d). (B) Cell death measured by MTT assay after 24 h of treatment is expressed as the percentage of cell death. Values are the mean ± SE of eight samples (*t test, P < 0.01). (C) Dose response of ghrelin in MTT cell death assay after 24 h of treatment. Values are the mean ± SE of eight samples. (D) Cell death measured by LDH release after 48 h of treatment. Values are the mean ± SE of eight samples (*t test, P < 0.01).