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. 2002 Dec 23;159(6):1029–1037. doi: 10.1083/jcb.200207165

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Copyright © 2002, The Rockefeller University Press

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Figure 3. — Ghrelin stimulates protein-tyrosine phosphorylation and activates ERK1/2 and Akt. Overnight serum-starved H9c2 cells were stimulated with 1 μM ghrelin and lysed at different times. Total lysates were analyzed by Western blot with antiphosphotyrosine antibodies (A), specific anti-phosphoERK1/2 antibodies (B, top) and anti-ERK1/2 antibodies (B, bottom), and specific anti-phosphoAkt antibodies (C, top) and anti-Akt antibodies (C, bottom). H9c2 cardiomyocytes (D) and PAE cells (E) were treated with 1 μM doxorubicin for 20 h (black bars) in the presence or absence of 1 μM ghrelin and in the presence or absence of 100 nM wortmannin or 30 μM PD98059. Cell death was measured by MTT assay. Values are the mean ± SE of eight samples (*t test, P < 0.01).