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. 2002 Dec 23;159(6):1029–1037. doi: 10.1083/jcb.200207165

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Copyright © 2002, The Rockefeller University Press

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Figure 5. — H9c2 cardiomyocytes express a ghrelin receptor distinct from GHSR-1a. (A and C) Specific binding was determined by incubation of crude membranes with increasing concentrations (0.25–20 nM) of either radio-labeled ghrelin (A) or radio-labeled des-acyl ghrelin (C) in the presence or absence of 1 μM unlabeled ghrelin (A) or unlabeled des-acyl ghrelin (C), respectively: total binding (▪), specific binding (▾), and nonspecific binding (○). Data are the average of duplicate assay determinants. Similar results were obtained in at least two other independent experiments. (B and D) Displacement curves of radio-labeled ghrelin (B) or radio-labeled des-acyl ghrelin (D) binding by unlabeled ghrelin (○), des-acyl ghrelin (▪), hexarelin (▴), and MK-0677 (▵). Displacement binding values are the mean ± SE of four separate experiments.