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. 2002 Dec 23;159(6):1029–1037. doi: 10.1083/jcb.200207165

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Copyright © 2002, The Rockefeller University Press

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Figure 6. — Des-acyl ghrelin inhibits cell death and activates ERK1/2 and Akt in H9c2 cardiomyocytes and PAE. (A) H9c2 cardiomyocytes and PAE cells were treated with 1 μM doxorubicin (grey bar) for 24 h in the presence or absence of either 1 μM ghrelin or des-acyl ghrelin. Cell death was assessed by the MTT assay. Values are mean ± SE of eight samples (*t test, P < 0.01). (B and C) H9c2 cardiomyocytes were serum starved overnight, stimulated with 1 μM des-acyl ghrelin, and lysed at different times. Total lysates were analyzed by Western blot with specific anti-phosphoERK1/2 antibodies (B, top) and anti-ERK1/2 antibodies (B, bottom) and specific anti-phosphoAkt antibodies (C, top) and anti-Akt antibodies (C, bottom).