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. 2002 Dec 23;159(6):1051–1059. doi: 10.1083/jcb.200210121

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Copyright © 2002, The Rockefeller University Press

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Figure 1. — Cdc37 overexpression suppresses v-Src activity defect in sti1 Δ yeast. (A) Western blot analysis using antiphosphotyrosine (top), anti–v-Src (middle), and antiphosphoglycerate kinase (bottom, Pak). Extracts were prepared after induction of v-Src for 6 h from wild type (lane 1) and sti1Δ strains minus and plus Cdc37 overexpression (lanes 2 and 3). Cells were grown at 25°C. (B) Extracts were prepared as describd for A, except that cells were grown at 30°C. (C) Pull-down assay with GST-Hsp90. V-Src was induced by growth of wild-type (WT) and sti1Δ cells in galactose-containing media. Lane 1, wild-type cells not expressing GST-hsp90; lane 2, wild-type cells expressing GST-Hsp90; lane 3, wild-type cells expressing GST-Hsp90 plus overexpressed Cdc37; lane 4, sti1Δ cells expressing GST-Hsp90; lane 5, sti1Δ cells expressing GST-Hsp90 plus overexpressed Cdc37. The bottom two panels show Western blots of proteins isolated on GST-agarose resin. The top two panels show Western blots of proteins in cell extracts.