Figure - PMC

Skip to main content

An official website of the United States government

Here's how you know

Here's how you know

Official websites use .gov
A .gov website belongs to an official government organization in the United States.

Secure .gov websites use HTTPS
A lock ( ) or https:// means you've safely connected to the .gov website. Share sensitive information only on official, secure websites.

View full-text article in PMC

. 2002 Aug 19;158(4):761–772. doi: 10.1083/jcb.200112050

Search in PMC
Search in PubMed
View in NLM Catalog
Add to search

Copyright © 2002, The Rockefeller University Press

This article is distributed under the terms of an Attribution–Noncommercial–Share Alike–No Mirror Sites license for the first six months after the publication date (see http://www.rupress.org/terms). After six months it is available under a Creative Commons License (Attribution–Noncommercial–Share Alike 4.0 Unported license, as described at http://creativecommons.org/licenses/by-nc-sa/4.0/).

PMC Copyright notice

Figure 5. — ETF1 is required for Cvt pathway trafficking. Cells were grown overnight at 26°C and pulse labeled with [³⁵S]methionine for 10 min at 38°C. (A) After a 30 min chase, labeled wild-type, vps34 ^tsf, and etf1Δ cells were lysed and immunoprecipitated for CPY. Samples were resolved by SDS-PAGE and fluorography. (B) After 1- or 2-h chases in the presence or absence of 0.2 μg/ml rapamycin, API immunoprecipitates derived from labeled wild-type, vps34 ^tsf, etf1Δ, and etf1 ^{(K113–118A)} strains were analyzed by SDS-PAGE and fluorography, revealing defects in API maturation in the etf1Δ cells by the Cvt pathway but not macroautophagy.