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. 2002 Aug 19;158(4):659–668. doi: 10.1083/jcb.200204081

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Copyright © 2002, The Rockefeller University Press

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Figure 5. — The cellular and subcellular distribution of Rab32. The tissue distribution of Rab32 mRNA was assessed by Northern blot analysis. (A) A human multi-tissue (tissue sources indicated above each lane) was screened using a 113-bp cDNA probe that corresponds to the COOH-terminal hypervariable region of Rab32. Hybridization was detected by autoradiography. The sizes of DNA markers are indicated. (B) Triton X-100–soluble extracts from the WI-38 human fetal lung fibroblasts were immunoblotted with affinity-purified anti-Rab32 antibody (left) or preimmune sera (right). Signals were detected by chemiluminescence. Molecular weight standards are indicated. Confocal immunofluorescence microscopy of WI-38 fibroblasts triple labeled with polyclonal antibodies against Rab32 (C, green), cell-permeable dye MitoTracker Red^TM (D, red), and a monoclonal antibody against α-tubulin (E, blue). A merged image (F) indicates the cellular distribution of all three signals. (G) Subcellular fractionation of WI-38 cells was performed according to the Materials and methods. Protein (20 μg each) from whole cell (W), nuclear (P1), and mitochondria-enriched (P2) fractions were subjected to SDS-PAGE and electrotransferred to nitrocellulose. Membranes were immunoblotted using affinity-purified polyclonal anti-Rab32 antibody (top) and a monoclonal anticytochrome oxidase subunit I (bottom) as a marker for mitochondria. Immunocytochemistry and confocal analysis were used to demonstrate the subcellular location of RII (H) and Rab32 (I). A merged image shows a significant overlap of the signals (J).