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. 2002 Aug 19;158(4):773–785. doi: 10.1083/jcb.200205049

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Copyright © 2002, The Rockefeller University Press

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Figure 1. — ERK activation by FSS and HOS. BAECs were exposed to 15 dyn/cm² of FSS or to culture medium containing 300 mM sucrose (HOS; ∼600 mOsm) for 0–20 min. Phospho-ERK, total ERK, and phospho-p38 were detected by immunoblotting using anti–phospho-ERK (pTpY-ERK1,2), anti-ERK (ERK1,2) and anti–phospho-p38 (pTpY-p38), respectively. The levels of ERK2 phosphorylation at 10 min over the control level were 2.1 ± 0.2 (n = 7) and 1.7 ± 0.1 (n = 6) times by HOS and FSS, respectively. p38 was activated by HOS but not by FSS.