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. 2002 Aug 19;158(4):731–740. doi: 10.1083/jcb.200202024

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Copyright © 2002, The Rockefeller University Press

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Figure 6. — Abrogation of REN expression reduces the levels of Neurogenin1 and NeuroD. (A) Western-blot of endogenous REN and protein encoded by transfected pCXN2-REN (REN) in P19 cells treated with RA, as described in B, and revealed using anti-REN antibody. (B) Northern blot analysis of RNA isolated from P19 cells stably transfected with either REN cDNA cloned in antisense orientation into pCXN2 (P19-REN-AS) or empty vector P19-pCXN2 and treated with RA as described in Fig. 1 A. Blots were hybridized with cDNA probes for neurogenin-1, NeuroD, Math1, Mash1, Id1, and Id3, as indicated on each panel. Ethydium bromide staining of ribosomal RNA is shown as a loading control.