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. 2002 Aug 19;158(4):669–679. doi: 10.1083/jcb.200204089

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Copyright © 2002, The Rockefeller University Press

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Figure 2. — Actin affects vacuole morphology. (A) Vacuole morphology of gene deletion or actin or LAS17 mutant strains. The strains RLY 574 (arp2Δ), DDY2266 (las17–16), GEY1290 (myo3Δ), GEY1090 (myo5Δ), GEY4840 (sac2Δ), GEY1290 (sac6Δ), GEY3370 (vrp1Δ), GEY2980 (cla4Δ), GEY3700 (arc18Δ), GEY0394–101 (act1–101), GEY0394–157 (act1–157), and the wild-type strain BY4742 (WT) were grown overnight in YPD, stained with FM4–46 and examined for vacuole morphology. The width of the field shown in each of the nine panels is 25 μm. (B) Immunoblot analysis of vacuoles and whole cell lysate prepared from strain GEY6024 for actin, actin signaling proteins (Vrp1p, Las17p, Arp3p, Cla4p, and Cdc42p), vacuolar proteins (Ypt7p and CPY), and a cytosolic chaperone (Ssa1p).