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. 2002 Aug 19;158(4):709–718. doi: 10.1083/jcb.200202002

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Copyright © 2002, The Rockefeller University Press

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Figure 4. — Recombination in cultured OL and efficiency of ablation of Notch1 in P0 Cnp-CreΔ/Δ spinal cord. Acutely dissociated cells isolated from individual P0 Cnp-Cre R26R mice homozygous for the floxed Notch1 allele (a and c, Δ/Δ) or from Cnp-Cre R26R heterozygous littermates (b and d, Δ/wt) were stained for X-gal (a and b) and O4 (c and d). Arrows mark double-labeled cells. (e) Southern blot analysis of DNA isolated from P0 spinal cord using a probe derived from the 5′ upstream region of the Notch1 locus revealed a 5.8-kb fragment from the wild-type allele after digestion with EcoRI. After recombination, a 2.3-kb fragment can be detected. Genotypes of the animals used are indicated above the lanes.