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. 2002 Aug 19;158(4):719–729. doi: 10.1083/jcb.200202124

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Copyright © 2002, The Rockefeller University Press

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Figure 3. — Female compound heterozygotes expressing rumpshaker and null alleles of the Plp gene, immunostained for PLP/DM20. Myelin sheaths formed by oligodendrocytes expressing the rumpshaker allele are positively stained, whereas those supported by cells expressing the null allele remain unstained. (A and B) Resin sections of ventral columns of spinal cord from mice aged P20 and P100, respectively. There is a small reduction in the number of positive sheaths between the two ages. Bars, 20 μm. (C and D) Resin sections of optic nerves from mice aged P20 and P50, respectively. Both mice show patches of rumpshaker myelin (arrows) comprising a minority of the myelin sheaths. Bars, 50 μm. (E and F) Paraffin sections of the dorsal columns of spinal cord from a heterozygote aged P200 (rsh/−) and her male rumpshaker littermate (rsh/Y), respectively. The affected male (F) indicates the amount of PLP/DM20 that can be present if all oligodendrocytes express the rumpshaker allele. The heterozygote contains considerably <50% positive sheaths although their concentration is higher in the corticospinal tracts (CST) than elsewhere in the dorsal columns. Bars, 50 μm. (G) Paraffin section of cerebral cortex from a heterozygote aged P200 to show several small regions containing rumpshaker myelin, suggesting single oligodendrocytes or a small clone of cells expressing the mutant allele. Bar, 100 μm. (H) Higher magnification of one of the patches of rumpshaker myelin shown in G. Bar, 20 μm.