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. 2002 Jun 10;157(6):1061–1070. doi: 10.1083/jcb.200203014

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Copyright © 2002, The Rockefeller University Press

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Figure 9. — Quantitative real time PCR analysis of annexin II, V, and VI, and type X collagen gene expression in chondrocytes treated with RA or RA/BAPTA. Total RNA was isolated from 1- to 6-d untreated, RA-, and RA/BAPTA-treated chondrocytes. Annexin II, V, and VI, and type X collagen gene expression were detected by quantitative real-time PCR using SYBR Green. PCR reactions were run in triplicates. Data were obtained from triplicated PCR reactions of three different cultures and values are mean ± SD (★, P ≤ 0.01, ★★, P ≤ 0.05 vs. 1-d untreated cultures.)