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. 2002 Jun 10;157(6):1029–1040. doi: 10.1083/jcb.200111012

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Copyright © 2002, The Rockefeller University Press

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Figure 2. — Phenotypes of cta4 Δ . (A) Disruption of cta4 ⁺ confers a temperature-sensitive phenotype and causes an imbalance in cation homeostasis. Cells were serially diluted in fivefold steps, spotted onto YES plates containing cations as indicated, and incubated for 3 d at 25, 30, or 36°C. Strains used were Fy1180, Hu185, and Hu285. (B) The effect of Ca²⁺ on the growth of the cta4Δ mutant. Growth curves showing the growth inhibition by different concentrations of CaCl₂ at 30°C.