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. 2002 Jun 10;157(6):963–974. doi: 10.1083/jcb.200111077

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Copyright © 2002, The Rockefeller University Press

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Figure 3. — NEM treatment of HeLa cellular lysates inhibits both RanGEF and RanGAP activity. Cellular lysates were prepared as described in the Materials and methods and treated with either (♦) 10, (▴) 20, (•) 40, or (▪) 60 mM NEM for the indicated period of time, quenched with excess DTT, and measured as follows: (A) measurement of RanGEF activity in HeLa cell extracts after NEM treatment; (B) measurement of RanGAP activity in HeLa cell extracts after NEM treatment. Both RanGAP and GEF activity were measured as described in the Materials and methods.

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