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. 2002 Jun 10;157(6):963–974. doi: 10.1083/jcb.200111077

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Copyright © 2002, The Rockefeller University Press

This article is distributed under the terms of an Attribution–Noncommercial–Share Alike–No Mirror Sites license for the first six months after the publication date (see http://www.rupress.org/terms). After six months it is available under a Creative Commons License (Attribution–Noncommercial–Share Alike 4.0 Unported license, as described at http://creativecommons.org/licenses/by-nc-sa/4.0/).

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Figure 6. — The entry of microinjected FITC–Kap-α into the nucleus is inhibited by 2-deoxyglucose/azide treatment. HeLa cells were incubated in either normal media or gluc⁻ media containing 2-deoxyglucose/azide for 20 min. They were then microinjected into the cytoplasm with either (A) TRITC–NLS-BSA (9.1 mg/ml) and a FITC 70-kD dextran marker (6.7 mg/ml) or (B) FITC–Kap-α (1.7 mg/ml) and a Cascade blue 70-kD dextran marker (0.9 mg/ml). After injection (which took ∼10 min), the cells were incubated for an additional 30 min in the treatment media before fixation.

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