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. 1983 Jan;153(1):543–545. doi: 10.1128/jb.153.1.543-545.1983

In Vitro Synthesis of Subunits of Bacterial Luciferase in an Escherichia coli System

Jillian F Evans 1, Susan McCracken 1,†, Carol M Miyamoto 1, Edward A Meighen 1, Angus F Graham 1
PMCID: PMC217406  PMID: 6184363

Abstract

RNA extracted from the luminescent bacterium Vibrio harveyi was translated in an Escherichia coli system. RNA from highly luminescent cells produced both α and β subunits of luciferase in vitro, as confirmed by immunoprecipitation and partial proteolysis. RNA from cells before induction of luminescence did not direct luciferase synthesis.

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Selected References

These references are in PubMed. This may not be the complete list of references from this article.

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