Figure 1.

Diagram of protocol for stretch-dependent binding of cytoplasmic proteins to Triton X-100–insoluble cytoskeletons. L-929 cells were cultured on a collagen-coated silicone substrate, and cytoskeletons were prepared by treating with 0.25% Triton X-100/ISO (+) buffer for 2 min (as described in Materials and methods). Triton X-100–insoluble cytoskeletons were either left unstretched or stretched (or relaxed from prestretch) with ISO (+) after washing three times. Then, the ISO (+) buffer was replaced with the cytoplasmic lysate solution (as described in Materials and methods), incubated for 2 min at room temperature, and washed four times with ISO (+) buffer.