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. 2002 Feb 18;156(4):595–602. doi: 10.1083/jcb.200110109

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Copyright © 2002, The Rockefeller University Press

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Figure 2. — RanGAP1 does not localize at the immediate vicinity of spindle poles. HeLa cells were stained with either anti-RanGAP1 or anti-RanBP2 and anti–γ-tubulin antibodies after permeabilization and fixation to observe the localization of these proteins at spindle poles. (A) γ-Tubulin (green) and RanGAP1 (red), as recognized by corresponding antibodies. (B) γ-Tubulin (green), RanBP2 (red), and DNA were visualized by staining with DAPI (blue).