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. 2002 Feb 18;156(4):653–664. doi: 10.1083/jcb.200110081

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Copyright © 2002, The Rockefeller University Press

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Figure 8. — Specific role of the Rab6a' isoform in EE/RE-to-TGN transport. (A) Sulfation analysis on intact cells overexpressing the indicated Rab6a and Rab6a′ mutants or a dominant negative Rab11 mutant. Note that dominant negative Rab6a′T27N strongly inhibited retrograde transport to the TGN. (B) Western blot analysis of the expression levels of the indicated Rab6 mutants. All mutants were equally well expressed. CTL, empty vector transfected cells. (C) Cy3-labeled STxB was internalized for 45 min at 37°C into cells expressing the indicated proteins. The cells were then fixed and stained with antibodies to the medial Golgi marker CTR433 or Rab6. (D) In Rab6a′T27N–expressing cells, STxB (red) accumulated in transferrin receptor (TfR, green) containing EE/RE.

Figure 8. — Specific role of the Rab6a' isoform in EE/RE-to-TGN transport. (A) Sulfation analysis on intact cells overexpressing the indicated Rab6a and Rab6a′ mutants or a dominant negative Rab11 mutant. Note that dominant negative Rab6a′T27N strongly inhibited retrograde transport to the TGN. (B) Western blot analysis of the expression levels of the indicated Rab6 mutants. All mutants were equally well expressed. CTL, empty vector transfected cells. (C) Cy3-labeled STxB was internalized for 45 min at 37°C into cells expressing the indicated proteins. The cells were then fixed and stained with antibodies to the medial Golgi marker CTR433 or Rab6. (D) In Rab6a′T27N–expressing cells, STxB (red) accumulated in transferrin receptor (TfR, green) containing EE/RE.