Table II. Characterization of pauses in droplet motion.
|
|
Pause duration
|
Time between pauses
|
Percentage reversals associated with a pause
|
|---|---|---|---|
| s | s | ||
| Pauses after minus-end travel | |||
| wild-type (*) | 0.62 ± 0.03 | 5.03 ± 0.31 | 13.8 |
| Dhc64C6-10/Dhc64C 8-1 (*) | 0.76 ± 0.03 | 4.24 ± 0.31 | 17.4 |
| Dhc64C6-10/+ | 0.62 ± 0.03 | 3.99 ± 0.29 | 14.2 |
| Dhc64C8-1/+ | 0.70 ± 0.03 | 2.98 ± 0.18 | 17.5 |
| Gl 1/+ | 0.64 ± 0.04 | 4.24 ± 0.31 | 13.4 |
| Pauses after plus-end travel | |||
| wild-type (*) | 0.55 ± 0.20 | 7.63 ± 0.45 | 11.7 |
| Dhc64C6-10/Dhc64C8-1 | 0.71 ± 0.03 | 3.98 ± 0.19 | 18.7 |
| Dhc64C6-10/+ | 0.60 ± 0.03 | 5.90 ± 0.32 | 12.7 |
| Dhc64C8-1/+ | 0.71 ± 0.04 | 3.94 ± 0.23 | 16.7 |
| Gl 1/+ | 0.65 ± 0.04 | 4.90 ± 0.30 | 13.7 |
Droplet motion in phase II embryos was characterized by centroid tracking. Pauses after minus-end or plus-end travel were recognized automatically by custom software and quantitated as described previously (Gross et al., 2000; Materials and methods). (First column) Average pause duration. (Second column) Time between pauses, a measure for how frequently pauses occur. (Third column) Fraction of reversals associated with a pause. This parameter indicates whether droplets typically pause first before reversing travel direction. In all cases, a majority of reversals occurs without a detectable pause. The data in rows labeled (*) are from our previous analysis of droplet travel (Gross et al., 2000) and are included for comparison.