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Effect of perfusion of AdCMV-OB-Rb or AdCMV-β-gal on the mRNA and protein of islet GLUT-2 mRNA semiquantified by RT-PCR and normalized for β-actin mRNA (A) and islet GLUT-2 protein quantified by immunoblotting (B). (C) GK mRNA semiquantitated by RT-PCR and normalized for β-actin mRNA. (D) GK protein quantified by immunoblotting. Results from three independent experiments with standard deviations depicted as error bar.
