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. 2008 Jan;19(1):17–29. doi: 10.1091/mbc.E07-05-0411

Figure 2.

Figure 2.

Actin may control early endosome exit in cells treated with the CaM antagonist. (A) NRK cells were incubated for 45 min with W13 (5 μg/ml) and 30 min with the actin-depolymerizing agent cytochalasin D (1 μM). Anti-EEA1 (Alexa Fluor 488) and phalloidin-TRITC stainings are shown. Bar, 10 μm. (B) Vero cells transiently expressing constitutively active YFP-cofilin (S3A) were treated with W13 for 60 min at 37°C. After fixation, the cells were immunolabeled with anti-EEA1 (Alexa Fluor 594) and phalloidin-TRITC. Bar, 10 μm.