Table 2.

Response to nutrient limitations in batch culture

Strain	Limiting nutrient	Supplement	Glucose 8 h	Consumed 24 h	% G0/G1 24 h
Prototroph	Phosphate	None	5.91	13.44	91
leu2	Phosphate	Excess leucine	5.04	10.15	93
ura3	Phosphate	Excess uracil	4.78	10.06	92
leu2	Leucine	None	10.22	18.70	60
ura3	Uracil	None	9.86	24.13	73

Batch cultures of isogenic CEN.PK derivatives were grown in chemostat minimal medium containing 1% glucose with limiting phosphate (10 mg/l), leucine (40 mg/l), or uracil (4 mg/l) supplements as described in Materials and Methods. Excess leucine and uracil, when provided, was 200 and 40 mg/l, respectively. The growth curves are shown in Supplemental Figure S3. Specific glucose consumption was calculated for time (t) as the normalized fraction of glucose consumed (grams per liter) using the equation ([glucose]_initial − [glucose]_t)/[glucose]_initial)/cells/ml.