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. 2008 Jan;19(1):65–77. doi: 10.1091/mbc.E07-06-0525

Figure 7.

Figure 7.

Cdk1 and p21 localize to the nucleus in the absence of Cdk2. Immunocytochemical costaining of Cdk1 and p21 in 5-Gy irradiated and serum-stimulated Cdk2+/+ and Cdk2−/− MEFs at the indicated time points. Cdk1 localization was detected by rabbit anti-Cdk1 antibodies followed by AlexaFluor568 goat anti-rabbit antibodies in Cdk2+/+ cells (red; B1–B5) and in Cdk2−/− cells (red; E1–E5). p21 was detected by mouse anti-p21 antibodies followed by AlexaFluor488 goat anti-mouse antibodies in Cdk2+/+ cells (green; C1–C5) and Cdk2−/− cells (green; F1–F5). The nuclei were counterstained with DAPI (blue; A1–A5 and D1–D5). The images were captured with a laser confocal microscope (63×). The representative pictures are from one of three independent stainings. Scale bar, 10 μm in all panels.