Figure 6.

SMOC-2 is dispensable for ligand-induced PDGFβR autophosphorylation and activation of MAPK and Akt. (A) Swiss 3T3 cells were transfected with siCon or siSMOC-2 oligonucleotide duplexes. The resulting cells were made quiescent by serum deprivation, and then they were stimulated with recombinant PDGF-BB (3 ng/ml) or were left untreated for controls. At different time points after PDGF-BB treatment, cell extracts were prepared and analyzed by SDS-PAGE and immunoblotting using antibodies against phospho-PDGFβR, PDGFR, phospho-MAPK, MAPK, phospho-Akt, and ILK. (B) Swiss 3T3 cells were transfected siCon or siSMOC-2 oligonucleotide duplexes. The resulting cells were made quiescent, and then they were treated with different concentrations of PDGFBB (0–100 ng/ml) and [³H]thymidine (1μCi/ml). Eighteen hours after stimulation, rates of DNA synthesis in the resulting cultures were determined using measurements of [³H]thymidine incorporation. Data points represent the mean for two samples, with error bars representing the range. *p < 0.001 compared with siCon-transfected cells.