Table 3.
Localization pattern of mRFP1-TeaA
| teaA(p)-mRFP1-TeaA |  |
||||
|---|---|---|---|---|---|
| WTa | 80 | 7 | 0 | 13 | 0 |
| WT with benomylb | 2 | 3 | 10 | 2 | 83 |
| ΔkipAc | 43 | 35 | 8 | 14 | 0 |
| KipA-rigord | 30 | 34 | 15 | 9 | 10 |
| cytochalasin Ae | 56 | 6 | 6 | 9 | 23 |
The localization pattern of 100—200 hyphal tips was analyzed and grouped into five different categories. The numbers indicate the percentage of hyphal tips of these groups.
SNT49 (a), SNT50 (c), and SNT51 (d) were grown on minimal medium with glycerol for 1 day. SNT49 (b) and SNT57 (e) were grown under the same conditions as above and treated with 2.5 μg/ml benomyl (b) or 2 μg/ml cytochalasin A (e) for 30 min. mRFP1-TeaA localization was analyzed within 10 min.