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. 2008 Jan;19(1):284–296. doi: 10.1091/mbc.E07-05-0519

Figure 4.

Figure 4.

Analysis of Muc1p abundance in filamentous growth mutants. (A) Dual reporter system describing the change in Muc1p-GFP abundance from wild type and the corresponding change in Yef3p-DsRed abundance. Data are presented here for those genes exhibiting changes in Muc1-GFP levels consistent with the observed filamentous growth phenotype without comparable changes in Yef3p-DsRed abundance. For comparison, the mdm30::mTn mutant and GCN4 overexpression mutant are included; Yef3p-DsRed levels differ significantly from wild-type in these mutants, suggesting that, in these cases, the observed changes in Muc1p-GFP levels likely represent general effects on protein production. For this analysis, error bars are generated from three biological replicates. (B) Studies of epistasis using MUC1 deletion and overexpression alleles in mutants disrupted for GLN3, WHI2, YGR050c, TEC1, and MDM30, and in mutants overexpressing ASP3-4, UGA3, YDR415c, and YJR008w. Filamentous growth phenotypes at the colony and cell levels are indicated, along with growth conditions for each analysis.