Figure 2.

Tap interacts with other human NXF family members. (A) The ability of the other NXF family members to interact with Tap was tested using a GST pull-down assay. In vitro synthesized ³⁵S-FLAG-Tap (lanes 1–3), ³⁵S-NXF2 (lanes 4–6), ³⁵S-NXF3 (lanes 7–9), and ³⁵S-FLAG-NXF5 (lanes 10–12) were incubated with glutathione-agarose beads coated with GST or GST-Tap. Bound proteins were resolved by SDS-PAGE followed by autoradiography. Input lanes correspond to 5% of radioactive protein used for each condition. (B) Using the same assay, ³⁵S-FLAG-NXF5 was tested for binding GST-NXF5 (lane 3) or GST alone (lane 2).