Figure 4.

The amino terminus of Tap is required for the Tap complex formation. In vitro translated Tap mutants were compared for binding to GST or GST-Tap WT immobilized onto glutathione-coated beads. Bound proteins were separated by SDS-PAGE and visualized by autoradiography. Input lanes correspond to 5% of radioactive protein loads. (A) Tap constructs with mutations in the NTF2-like or UBA domains. (B) Tap deletion constructs.