Figure 5.
Oligomerized Tap interacts with Nxt1. (A) In vitro-translated 35S-FLAG-Tap (lanes 1, 4, and 6), 35S-Nxt1 (lanes 2, 5, and 7), or 35S-FLAG-Tap together with 35S-Nxt1 (lanes 3 and 8) were incubated with GST or GST-Tap F513D beads. Bound proteins were separated by SDS-PAGE and visualized by autoradiography. Input lanes correspond to 5% of radioactive protein loads. (B) Graphic representation of the above-mentioned experiment showing that the GST-Tap F513D mutant, unable to bind Nxt1 directly, can recruit 35S-Nxt1 indirectly when in a multimeric complex with 35S-FLAG-Tap WT.