Figure 3.

Three-dimensional reconstructions of γ-TuSC. Volumes were generated with tilted particles corresponding to classes 1, 2, and 3 in Figure 1C. Four orthogonal views of each particle are shown, and the rotations between the views are indicated at left. The aligned structures are superimposed at right, with the constant region used for the alignment rendered semitransparent. The arm on the left in the top view adopts different orientations in the three structures, and it is rotated relative to the arm in class 3 by 8° in class 2 and by 15° in class 1.