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Labeling of the Tub4p N-terminus. (A) Individual particles of γ-TuSC with an N-terminal 6xHis-tag on Tub4p incubated with Ni2+-Nanogold. (B) Average of 68 labeled particles. (C) Average of 68 unlabeled particles. (D) Difference map generated by subtracting the labeled average from the unlabeled average. The prominent peak corresponding to the location of the gold label is 9.3σ above the mean. Bar, 10 nm.
