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. Author manuscript; available in PMC: 2008 Jan 3.
Published in final edited form as: Cell. 2007 Nov 30;131(5):940–951. doi: 10.1016/j.cell.2007.09.037

Figure 4. MRP4 Inhibition Enhances Intracellular cAMP Signals.

Figure 4

(A) The monochrome CFP image showing cytosolic distribution of the fluorescent EPAC probe in T84 cells transfected with CFP-EPAC-YFP; and representative pseudocolor images of CFP/FRET emission ratio before (time = 0 min) and after the addition of 20 μM MK571 and/or 2 μM ADO (time = 5 min). The images in each panel were captured from the same field of view. Color bar shows magnitude of the emission ratio. Scale bar: 10 μm.

(B) Kinetics of cAMP changes (represented by the normalized CFP/FRET emission ratio) recorded in the cells shown in (A). Arrow indicates addition of the reagents.

(C) CFP image and representative pseudocolored CFP/FRET ratio images before (time = 0 min) and after the addition of 20 μM MK571 and/or 100 μM ADO (time = 5 min). The probe was excluded from the nuclear compartments, although in these nonconfocal images, a signal emanating from above and below the nucleus gives the appearance of a nuclear ratio change. Scale bar: 10 μm.

(D) Kinetics of cAMP changes (normalized CFP/FRET emission ratio) recorded in the cells shown in (C). Arrow indicates addition of the reagents.

(E) The summary of all the experiments performed in the same conditions as in (A)-(D) and in Figure S5. Data represent the mean ± SEM (n=4–6).