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. 1999 Oct 18;147(2):375–388. doi: 10.1083/jcb.147.2.375

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© 1999 The Rockefeller University Press

This article is distributed under the terms of an Attribution–Noncommercial–Share Alike–No Mirror Sites license for the first six months after the publication date (see http://www.rupress.org/terms). After six months it is available under a Creative Commons License (Attribution–Noncommercial–Share Alike 4.0 Unported license, as described at http://creativecommons.org/licenses/by-nc-sa/4.0/).

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The basal membrane assay. An E6 retina was first dissected and flatmounted on a nitrocellulose membrane filter (MF), and then put upside down onto a PLL-coated glass coverslip, weightened, and incubated for 10 min (A). After lifting the filter up the basal membrane (BM) sticks to the surface of the glass coverslip (B) (Scheme after Kroeger 1989).