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. 1999 Oct 18;147(2):375–388. doi: 10.1083/jcb.147.2.375

Figure 4.

Figure 4

A shows the α1-AP fusion protein in cos7 cell supernatant, immunodetected with IG2 sera. α1-AP consists of the extracellular domain of CRYPα, which has a molecular weight of ∼90 kD together with the alkaline phosphatase of 65 kD. B and C show binding of the α1-AP fusion protein to a section of the E6 retina, visualized with AP staining. C is the corresponding phase contrast view. Arrows indicate staining in the BM of the retina. The arrowheads indicate the pigment epithelium. D shows binding of the α1-AP fusion protein to a BM from a E7 retina, visualized with AP staining. The stained dots represent glial endfeet. There is no binding of α1-AP to an E7 detergent-washed endfeet-free BM detectable as can be seen in E. Abbreviations: BM, basal membrane; Ret, retinal cell layer; PE, pigmented epithelium. Bars: (B and C) 0.1 mm; (D and E) 0.01 mm.