Table 1.

Statistical Analysis of Growth Cone Morphology

	Substrate + antibody		n	Growth cone diameter ± SEM	P
				μm
BM	Control	d1	60	12.86 ± 2.59	vs. BM-Ef, <0.0001
		d2	60	11.24 ± 2.08	vs. BM-Ef, <0.0001
BM	20 μg NCAM	d1	40	12.22 ± 1.63	NS
		d2	40	10.98 ± 1.49	NS
BM	20 μg IG2	d1	40	11.74 ± 1.36	NS
		d2	40	5.14 ± 1.36	<0.0001
BM	20 μg β-integrin	d1	40	7.07 ± 4.93	<0.0001
		d2	40	3.75 ± 2.85	<0.0001
LN	Control	d1	40	15.22 ± 2.23	vs. BM, 0.0014
		d2	40	13.23 ± 2.54	vs. BM, 0.0016
LN	20 μg NCAM	d1	40	13.99 ± 2.22	NS
		d2	40	14.96 ± 1.95	NS
LN	20 μg IG2	d1	40	12.95 ± 2.83	0.0059
		d2	40	12.96 ± 3.31	NS
LN	20 μg β-integrin	d1	40	Not detectable
		d2	40	Not detectable
BM-Ef	Control	d1	40	17.44 ± 1.96	vs. LN, 0.0002
		d2	40	16.41 ± 2.88	vs. LN, <0.0001
BM-Ef	20 μg NCAM	d1	40	17.61 ± 2.94	NS
		d2	40	16.26 ± 2.26	NS
BM-Ef	20 μg IG2	d1	40	15.32 ± 2.39	0.0163
		d2	40	15.35 ± 2.08	NS
BM-Ef	20 μg β-integrin	d1	40	5.40 ± 5.96	<0.0001
		d2	40	2.59 ± 2.84	<0.0001

n, Number of measured growth cones (10 growth cones from 4/6 independent experiments were analyzed); P, values from single-factor ANOVA analyzing the influence of IG2 on the same substrate and the influence of the different substrates themselves, 99% confidence interval NS indicates, no significance (statistical values > 0.05).