Table 2.
Statistical Analysis of the Average Axon Length
| Substrate + antibody | n | Average axon length ± SEM | P | ||
|---|---|---|---|---|---|
| P1 | P2 | ||||
| μm | |||||
| BM | Control | 19 | 642.0 ± 83.4 | — | vs. LN, NS |
| BM | 20 μg NCAM | 6 | 634.0 ± 93.7 | NS | vs. LN, NS |
| BM | 20 μg IG2 | 16 | 232.4 ± 27.3 | <0.0001 | vs. LN, <0.0001 |
| BM | 20 μg β-integrin | 9 | 184.2 ± 57.0 | <0.0001 | vs. LN, 0.0001 |
| LN | Control | 14 | 635.5 ± 85.5 | — | vs. BM-Ef, <0.0001 |
| LN | 20 μg NCAM | 8 | 698.2 ± 76.1 | NS | vs. BM-Ef, NS |
| LN | 20 μg IG2 | 9 | 442.8 ± 53.1 | <0.0001 | vs. BM-Ef, <0.0001 |
| LN | 20 μg β-integrin | 5 | 20.4 ± 25.8 | <0.0001 | vs. BM-Ef, 0.0004 |
| BM-Ef | Control | 8 | 1073.6 ± 209.3 | — | vs. BM, <0.0001 |
| BM-Ef | 20 μg NCAM | 8 | 1014.8 ± 119.4 | NS | vs. BM, <0.0001 |
| BM-Ef | 20 μg IG2 | 13 | 796.6 ± 94.3 | 0.0015 | vs. BM, <0.0001 |
| BM-Ef | 20 μg β-integrin | 6 | 220.6 ± 69.7 | <0.0001 | vs. BM, NS |
| MG | Control | 9 | 970.4 ± 173.3 | — | vs. BM-Ef, NS |
| MG | 20 μg NCAM | 3 | 883.8 ± 65.7 | NS | vs. BM-Ef, NS |
| MG | 20 μg IG2 | 11 | 909.8 ± 109.7 | NS | vs. BM-Ef, 0.014 |
| MG | 20 μg β-integrin | 7 | 470.5 ± 74.4 | <0.0001 | vs. BM-Ef, 0.001 |
| NC/PLL | Control | 9 | 281.5 ± 35.9 | — | — |
| NC/PLL | 20 μg NCAM | 3 | 256.8 ± 30.9 | NS | — |
| NC/PLL | 20 μg IG2 | 6 | 252.7 ± 41.3 | NS | — |
| NC/PLL | 20 μg β-integrin | 3 | 213.4 ± 5.8 | 0.0014 | — |
n, Number of independent experiments with at least two retinal strips; P1, values from single-factor ANOVA (ANOVA was used because most of the data show a normal distribution) analyzing the influence of different antibodies on the same substrate, 99% confidence interval; P2, values from single-factor ANOVA analyzing the influence of the growth substrate when treated with the same antibody, 99% confidence interval (the substrate that was used for the comparison is indicated); NS indicates no significance (statistical values > 0.05).