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. 1999 Oct 18;147(2):401–416. doi: 10.1083/jcb.147.2.401

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© 1999 The Rockefeller University Press

This article is distributed under the terms of an Attribution–Noncommercial–Share Alike–No Mirror Sites license for the first six months after the publication date (see http://www.rupress.org/terms). After six months it is available under a Creative Commons License (Attribution–Noncommercial–Share Alike 4.0 Unported license, as described at http://creativecommons.org/licenses/by-nc-sa/4.0/).

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graphic file with name JCB9902056.f10a.jpg — A possible role for RhoA, Cdc42, MKK3, and MKK4 in integrin α2β1-mediated activation of p38α. Equal numbers of stable α2-transfected Saos-2 cells were transiently cotransfected with flag-tagged p38α (2 μg) and the dominant negative form of the protein indicated (4 μg). The cells were treated and assayed for p38 kinase activity. The possible effect of the cotransfected plasmid on p38α-flag expression was checked with Western blot analysis of the cell lysate using flag-tag recognizing antibody M2. An autoradiogram of a representative experiment is shown. (B) Various concentrations of dominant negative (DN) Cdc42 were tested in cotransfections with 2 μg of p38α-flag. Each experiment was repeated 2 to 4 times and mean values ± SEM are shown. Transfection of wild-type (wt) Cdc42 was used as a control.

graphic file with name JCB9902056.f10b.jpg — A possible role for RhoA, Cdc42, MKK3, and MKK4 in integrin α2β1-mediated activation of p38α. Equal numbers of stable α2-transfected Saos-2 cells were transiently cotransfected with flag-tagged p38α (2 μg) and the dominant negative form of the protein indicated (4 μg). The cells were treated and assayed for p38 kinase activity. The possible effect of the cotransfected plasmid on p38α-flag expression was checked with Western blot analysis of the cell lysate using flag-tag recognizing antibody M2. An autoradiogram of a representative experiment is shown. (B) Various concentrations of dominant negative (DN) Cdc42 were tested in cotransfections with 2 μg of p38α-flag. Each experiment was repeated 2 to 4 times and mean values ± SEM are shown. Transfection of wild-type (wt) Cdc42 was used as a control.