Figure - PMC

Skip to main content

An official website of the United States government

Here's how you know

Here's how you know

Official websites use .gov
A .gov website belongs to an official government organization in the United States.

Secure .gov websites use HTTPS
A lock ( ) or https:// means you've safely connected to the .gov website. Share sensitive information only on official, secure websites.

View full-text article in PMC

. 1999 Oct 18;147(2):401–416. doi: 10.1083/jcb.147.2.401

Search in PMC
Search in PubMed
View in NLM Catalog
Add to search

© 1999 The Rockefeller University Press

This article is distributed under the terms of an Attribution–Noncommercial–Share Alike–No Mirror Sites license for the first six months after the publication date (see http://www.rupress.org/terms). After six months it is available under a Creative Commons License (Attribution–Noncommercial–Share Alike 4.0 Unported license, as described at http://creativecommons.org/licenses/by-nc-sa/4.0/).

PMC Copyright notice

graphic file with name JCB9902056.f2a.jpg — Characterization of the cellular location of α2 and α2/α1 integrin and formation of actin stress fibers. Stable Saos-2 transfectants were either grown in DME 10% FCS on immunofluorescence glass slides, plated on collagen coated glass slides, and allowed to adhere for 4 h in DME (serum-free) or grown inside a three-dimensional collagen gel for 48 h. (A) After incubation, the cells were fixed and stained for α2 integrin by using mAb followed by FITC-labeled rabbit anti–mouse antibody. Cells were washed, mounted, and examined on a microscope: (a) α2 clone 47 and (b) α2/α1 clone 18 on serum-derived fibronectin and vitronectin; (c) α2 clone 47 and (d) α2/α1 clone 18 on collagen; (e) α2 clone 47 and (f) α2/α1 clone 18 inside collagen gel; and (g) vector clone as a negative control. (B) Cells were stained for actin using fluorescein-conjugated phalloidin (a) α2 clone 47 and (b) α2/α1 clone 18 on collagen.

graphic file with name JCB9902056.f2b.jpg — Characterization of the cellular location of α2 and α2/α1 integrin and formation of actin stress fibers. Stable Saos-2 transfectants were either grown in DME 10% FCS on immunofluorescence glass slides, plated on collagen coated glass slides, and allowed to adhere for 4 h in DME (serum-free) or grown inside a three-dimensional collagen gel for 48 h. (A) After incubation, the cells were fixed and stained for α2 integrin by using mAb followed by FITC-labeled rabbit anti–mouse antibody. Cells were washed, mounted, and examined on a microscope: (a) α2 clone 47 and (b) α2/α1 clone 18 on serum-derived fibronectin and vitronectin; (c) α2 clone 47 and (d) α2/α1 clone 18 on collagen; (e) α2 clone 47 and (f) α2/α1 clone 18 inside collagen gel; and (g) vector clone as a negative control. (B) Cells were stained for actin using fluorescein-conjugated phalloidin (a) α2 clone 47 and (b) α2/α1 clone 18 on collagen.