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. 1999 Oct 18;147(2):351–366. doi: 10.1083/jcb.147.2.351

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© 1999 The Rockefeller University Press

This article is distributed under the terms of an Attribution–Noncommercial–Share Alike–No Mirror Sites license for the first six months after the publication date (see http://www.rupress.org/terms). After six months it is available under a Creative Commons License (Attribution–Noncommercial–Share Alike 4.0 Unported license, as described at http://creativecommons.org/licenses/by-nc-sa/4.0/).

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HSET is expressed as two isoforms in HeLa cells. Total HeLa cell protein was separated by SDS-PAGE and then was blotted with the HSET-specific antibody (αHSET-1) or a COOH-terminal peptide antibody (αCTP-2; Walczak et al. 1997). The two isoforms of HSET detected by αHSET-1 are designated HSET-H and HSET-L. Migration positions of myosin (200), β-galactosidase (116), phosphorylase B (97), and albumin (66) are shown in kD.