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. 1999 Oct 18;147(2):351–366. doi: 10.1083/jcb.147.2.351

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© 1999 The Rockefeller University Press

This article is distributed under the terms of an Attribution–Noncommercial–Share Alike–No Mirror Sites license for the first six months after the publication date (see http://www.rupress.org/terms). After six months it is available under a Creative Commons License (Attribution–Noncommercial–Share Alike 4.0 Unported license, as described at http://creativecommons.org/licenses/by-nc-sa/4.0/).

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graphic file with name JCB9906135.f3ab.jpg — HSET is essential for microtubule organization in metaphase II spindles in mouse oocytes. Mouse oocytes at the germinal vesicle stage were either mock injected (A), injected with antibodies specific for HSET (Β and C), or injected with antibodies specific for Eg5 (D). Injected oocytes were then matured in vitro until metaphase I (7 h; B) or until metaphase II arrest (16 h; A, C, and D). Oocytes were processed for indirect immunofluorescence using antibodies specific for tubulin (red), DNA (blue), and either Eg5 (A) or the injected antibody (B–D; green). Arrows indicate cytoplasmic asters; arrowheads indicate foci of HSET antigen; and the first polar bodies are marked when discernible (1^stPB). E, Percentage of microinjected oocytes that mature to metaphase II arrest after 16 h of maturation in vitro postinjection. Oocytes were either mock injected, injected with antibodies specific for Eg5 antibody, or injected with antibodies specific for HSET, as indicated. Bar, 10 μm.

graphic file with name JCB9906135.f3cd.jpg — HSET is essential for microtubule organization in metaphase II spindles in mouse oocytes. Mouse oocytes at the germinal vesicle stage were either mock injected (A), injected with antibodies specific for HSET (Β and C), or injected with antibodies specific for Eg5 (D). Injected oocytes were then matured in vitro until metaphase I (7 h; B) or until metaphase II arrest (16 h; A, C, and D). Oocytes were processed for indirect immunofluorescence using antibodies specific for tubulin (red), DNA (blue), and either Eg5 (A) or the injected antibody (B–D; green). Arrows indicate cytoplasmic asters; arrowheads indicate foci of HSET antigen; and the first polar bodies are marked when discernible (1^stPB). E, Percentage of microinjected oocytes that mature to metaphase II arrest after 16 h of maturation in vitro postinjection. Oocytes were either mock injected, injected with antibodies specific for Eg5 antibody, or injected with antibodies specific for HSET, as indicated. Bar, 10 μm.