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. 1999 Oct 18;147(2):389–400. doi: 10.1083/jcb.147.2.389

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© 1999 The Rockefeller University Press

This article is distributed under the terms of an Attribution–Noncommercial–Share Alike–No Mirror Sites license for the first six months after the publication date (see http://www.rupress.org/terms). After six months it is available under a Creative Commons License (Attribution–Noncommercial–Share Alike 4.0 Unported license, as described at http://creativecommons.org/licenses/by-nc-sa/4.0/).

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Pertussis toxin stimulates MAP kinase in SMC. SMC were treated with pertussis toxin (PTX) at the concentrations indicated overnight, and lysed directly in RIPA buffer. Cell lysates were subjected to immunoprecipitation using anti-ERK antibody. Kinase assays were performed as described in Materials and Methods. Data are MAP kinase activity ±SD.