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. 1999 Oct 18;147(2):335–350. doi: 10.1083/jcb.147.2.335

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© 1999 The Rockefeller University Press

This article is distributed under the terms of an Attribution–Noncommercial–Share Alike–No Mirror Sites license for the first six months after the publication date (see http://www.rupress.org/terms). After six months it is available under a Creative Commons License (Attribution–Noncommercial–Share Alike 4.0 Unported license, as described at http://creativecommons.org/licenses/by-nc-sa/4.0/).

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Spindles and nuclei mislocalize in kar3-ts dyn1Δ cells. The nucleus and spindle were drawn away from the proper position at the mother–bud neck after loss of Kar3p function in the absence of dynein, but not in the absence of dynein and Kip2p. (A) kar3Δ dyn1Δ (pkar3-64) cells (MAY5400) were arrested with hydroxyurea at 26°C and then shifted to 35°C for 3 h in the continued presence of hydroxyurea. Samples taken before (top row) and after (bottom row) the temperature shift were processed for antitubulin immunofluorescence (left column) and DAPI staining of DNA (right column). (B) kar3Δ dyn1Δ kip2Δ (pkar3-64) cells (MAY5465) were treated as in A.