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. 1999 Oct 18;147(2):335–350. doi: 10.1083/jcb.147.2.335

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© 1999 The Rockefeller University Press

This article is distributed under the terms of an Attribution–Noncommercial–Share Alike–No Mirror Sites license for the first six months after the publication date (see http://www.rupress.org/terms). After six months it is available under a Creative Commons License (Attribution–Noncommercial–Share Alike 4.0 Unported license, as described at http://creativecommons.org/licenses/by-nc-sa/4.0/).

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Spindle defects caused by loss of Kar3p function. Cell cultures of the genotypes indicated were synchronized with α-factor at 26°C and then released into medium at 35°C. After 60 min, samples were processed for antitubulin immunofluorescence microscopy. Microtubules of six representative cells are shown for each genotype. Note that the top left example of the kar3Δ kip3Δ (pkar3-64) strain represents a rare bipolar spindle observed for cells of this genotype. Strains used are listed in legend of Fig. 1.